ISOLASI DAN IDENTIFIKASI SENYAWA YANG BERSIFAT ANTIBAKTERI DAN TOKSIK DALAM TUMBUHAN ILER {Coleiis scutellarioides [L.] Benth)

Dionisius  Tungga; I Made Dira  Swantara; Yenni Ciawi

doi:10.37478/agr.v1i2.484

Authors

Dionisius Tungga Fakultas Pertanian Universitas Flores
I Made Dira Swantara Fakultas MIPA Universitas Udayana
Yenni Ciawi Fakultas Teknik Universitas Udayana

DOI:

https://doi.org/10.37478/agr.v1i2.484

Abstract

The isolation and identification of antibacterial and toxic compound on Coleiis scutellarioides [L.] Benth. has been conducted. The extraction applied was maceration method using ethanol 85 %. The ethanol extract then partitioned using three kinds of solvents namely n-hexane, chloroform, ethyl acetate respectively. The most toxic and active antibacterial extraction was separated by chromatography column with n-hexane: chloroform as the mobile phase. Identification of the active isolate was conducted by gas chromatography-mass spectroscopy with the aim of'Wiley 229.L database. Toxic test and antibacterial activity test showed that FB₂ was proved to be the most toxic and active antibacterial one. The most toxic and active antibacterial fraction of FB₂ showed a retardation area of 66,98 mm² to Micrococcus luteus and 54,43 mm² to Escherichia coli, and LC₅₀ at 90,72 ppm. Gas chromatography-mass spectroscopy data show that the FB2 fraction contains 4 compounds namely palmitate acid (24,41%); stearate acid (19,53%); 9-oktadekenamida (22,95%); and Esther dioctyl hexadioat (33,11 %»)•

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Keywords:

Isolation, Identification, Antibacterial compounds, Toxic compounds

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